Application

Anti-Interleukin-1β Antibody is an antibody against Interleukin-1β for use in ELISA, WB, NEUT.

ELISA: 0.5 µg/mL this dilution will allow detection (in direct ELISA) of

0.2-0.4 ng/well when rat IL-1(is used to coat the plate.

Western blot: 0.1-0.2 µg/mL, this dilution should offer detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.

Neutralization: to yield ½ maximal inhibition (ND50) of the biological activity of rat IL-1beta (0.2 ng/mL), a concentration of 0.09-0.14 µg/mL of antibody is required.

Final working dilutions must be determined by end user.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly pure (>98%) recombinant rat IL-1beta.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-462; MABF22

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified Immunoglobulin. Lyophilized from 0.5X PBS pH 7.4, no preservatives. Reconstitute in sterile distilled water.

Specificity

Reacts with rat Interleukin-1beta (IL-1beta).

Storage and Stability

Maintain lyophilized material desiccated below 0°C. After reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.